It has been reported that in the regions of Dumfriesshire, three cases of E. coli have been confirmed. It is assumed that the three got affected with the disease while participating in the Tough Mudder event.

The Tough Mudder event was held in July and attracted 6,000 participants. The event was held at Drumlanrig Castle. The cases of E. coli have also been reported in other regions of Scotland. Following the confirmed cases of E. coli, the health authorities have sent alert all over the UK.

The patients affected with the disease are getting treatment from the specialist of Scotland. During the alleged game, the participants got massed up with the mud. They were challenged to navigate their ways after crossing muddy path and ice water.

Health Protection Scotland's (HPS) spokesperson said, ".. the local authority have been investigating possible sources of infection, there are inevitable limitations to investigation after large, one-off international events such as this one".

Authorities are of the view that continuous contact with the mud might have made the participants vulnerable to E. coli. The recent incidence has emphasized a need of making participants aware about the risk involved in taking part in activities like this one.